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puc t vector  (TaKaRa)


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    Structured Review

    TaKaRa puc t vector
    Puc T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc+t+vector/pmc11311731-241-18-20
    Average 86 stars, based on 1 article reviews
    puc t vector - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecular cloning of adipose triglyceride lipase (ATGL) gene from blunt snout bream and its expression after LPS-induced TNF-α factor.
    Article Snippet: The aims of the present study were to clone the full-length cDNA of adipose triglyceridelipase (ATGL) and to analyze its expression after lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α).. The cDNA obtained covered 1801 bp with an open reading frame of 1500 bp encoding 499 amino acids.. Sequence alignment and phylogenetic analysis show the best identity with Cyprinus carpio (86%).

    Plasmid Preparation:

    Article Title: Molecular cloning of adipose triglyceride lipase (ATGL) gene from blunt snout bream and its expression after LPS-induced TNF-α factor.
    Article Snippet: The aims of the present study were to clone the full-length cDNA of adipose triglyceridelipase (ATGL) and to analyze its expression after lipopolysaccharide (LPS)-induced tumor necrosis factor alpha (TNF-α).. The cDNA obtained covered 1801 bp with an open reading frame of 1500 bp encoding 499 amino acids.. Sequence alignment and phylogenetic analysis show the best identity with Cyprinus carpio (86%).

    Purification:

    Article Title: Metabolome and Transcriptome Unveil the Correlated Metabolites and Transcripts with 2-acetyl-1-pyrroline in Fragrant Rice
    Article Snippet: .. DNA fragments were purified using a TIANgel Midi purification kit (Tiangen, Beijing, China) and then cloned into a pUC-T vector (Takara, Dalian, China) for further Sanger sequencing (Tsingke, Guangzhou, China). .. The sequences were assembled using ContigExpress software v. 11.3 (Invitrogen, Waltham, MA, USA).

    Clone Assay:

    Article Title: Metabolome and Transcriptome Unveil the Correlated Metabolites and Transcripts with 2-acetyl-1-pyrroline in Fragrant Rice
    Article Snippet: .. DNA fragments were purified using a TIANgel Midi purification kit (Tiangen, Beijing, China) and then cloned into a pUC-T vector (Takara, Dalian, China) for further Sanger sequencing (Tsingke, Guangzhou, China). .. The sequences were assembled using ContigExpress software v. 11.3 (Invitrogen, Waltham, MA, USA).

    Sequencing:

    Article Title: Metabolome and Transcriptome Unveil the Correlated Metabolites and Transcripts with 2-acetyl-1-pyrroline in Fragrant Rice
    Article Snippet: .. DNA fragments were purified using a TIANgel Midi purification kit (Tiangen, Beijing, China) and then cloned into a pUC-T vector (Takara, Dalian, China) for further Sanger sequencing (Tsingke, Guangzhou, China). .. The sequences were assembled using ContigExpress software v. 11.3 (Invitrogen, Waltham, MA, USA).



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